تصلب الجلد Scleroderma: متلازمة كريست, صباغ القسيمات المركزية CREST syndrome, centromere staining

Centromere immunofluorescent staining of a human lymphoid cell line is present in this patient with the CREST syndrome. It is demonstrated by using either a chromosome spread technique or a human laryngeal carcinoma (HEp-2) cell line as a substrate for indirect immunofluorescence of antinuclear antibody. The centromere staining is characterized by discrete nuclear speckles. The number of speckles in the nucleus is proportional to the diploid chromosome number of the cell used as a substrate. This pattern of nuclear staining is found primarily in the CREST and is described as occurring in 57% to 98% of patients. Centromere staining has also been demonstrated in about 10% of patients with systemic sclerosis and in about 20% of patients with Raynaud's disease.